Advantages
- Using a culture medium that removes methionine and controls the zinc content allows for the elimination of undifferentiated cells and the promotion of stem cell differentiation.
- Utilizing a culture medium that controls the levels of insulin-like growth factors and zinc can promote the differentiation of insulin-producing cells from pluripotent cells.
Background and Technology
ES cells and iPS cells are attracting attention as important sources for regenerative medicine and are expected to be induced into specific organ cells. However, problems have been that undifferentiated stem cells remain or are mixed in when inducing differentiation into various types of cells, and that there are differences in the differentiation efficiency of each stem cell.
Researchers discovered that by culturing cells in a methionine-depleted medium before culturing them in a differentiation-inducing medium, they could remove undifferentiated cells and promote differentiation. Furthermore, since intracellular zinc promotes the proliferation of undifferentiated cells and inhibits differentiation after culture in a methionine-depleted medium, by using a medium that combines methionine and zinc removal, We have discovered a highly versatile differentiation induction method that eliminates undifferentiated cells and promotes differentiation.
Data
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Reference and Patent
JP5938726, WO2015/125662, WO2019/208713
Principal Investigator
Shoen Kume (Tokyo Institute of Technology, School of Life Science and Technology, Professor)
Expectations
The researchers look forward to collaborating with companies that are considering commercializing culture media using this technology, or those that wish to use it within their own companies. It is also possible to have an interview with the professor, so if you are interested, please contact us.
Project.DD-04902