Advantages
- Available for large scale of dendritic cells (DC) or macrophages (MP)
- Long-prolonged proliferation period; minimum period is 3 months
- Dendritic cells derived from Myeloid Cell Line could be model cells for virus research
- Further established interferon-β/interferon-γ expressing iPS-ML for cell therapy
Background & Technology
Myeloid blood cells are a group of cells classified within leukocytes and include macrophages, dendritic cells, and granulocytes. Macrophages and dendritic cells are used as cell therapy, and to achieve clinical efficacy, large numbers of cells are needed to achieve clinical efficacy. Until now, no method has been reported that can sustain proliferation for a long period of time (more than one month) and produce large numbers of myeloid blood cells, because it required considerable labor and time.
This technology is used to induce the forcible expression of various genes in ML derived from pluripotent stem cells (iPSCs) or peripheral blood monocytes, thereby adding the ability for long-term proliferation. Specifically, this is a method for producing ML from iPSCs (iPS-ML) or Monocytes (Monocytes-ML) with proliferation capability which includes the forcible expression of cMYC and the other exogenous gene.
Inventor established a method of xeno-free differentiation culture to generate a large scale of functional dendritic cells (iPS-DC) /macrophages (iPS-MP), Monocytes-DC/macrophages (Monocytes-MP) and demonstrated their potential for cell-therapy, drug screening or research application.
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Outline of method of producing Myeloid blood cell lines
Data
Proliferative myeloid cells derived from iPS cells (iPS-ML)
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Morphology of iPS-ML derived dendritic cell like cells generated by forced expression of cMYC and BMI1
Patent
JP5861191, US10,034,900
Publication
[1] Haruta et l. (2013) Gene Ther. May;20(5):504-13.
[2] Haruta et al. (2013) Hum Immnol.;74(10):1400-8.
[3] Koba et al. (2013) PLos One ;8(6): e67567
[4] Dao Huy Manh et al. (2018) J Gen Virol.;2018 Sep;99(9):1239-1247
Applicant
National University Kumamoto University
Expectation
We are looking for partner companies interested in license-in and utilizing this method and technology. We would be happy to start with a detailed explanation and discussion of the technology.
Project No. ON-00721