Neuro-protectant Peptides for Ischemic Stroke

Fragmentation of RANKL Eliminates Side-effect while Boosting Effect.

Core Benefit

  • First-in-class drug with new mechanism of action.
  • Potentially also effective for sepsis by controlling excessive inflammatory response, and osteoporosis by blocking osteoclast differentiation.

Background and Technology

Recent studies have shown that administration of recombinant receptor activator of nuclear factor κB (NF-κB) ligand (RANKL) reduces infarct volume and brain edema in ischemic stroke with reduced expression in inflammatory cytokines through a Toll-like receptor 2/4 (TLR2/4) signaling pathway in microglia [1]. However, RANKL also activated osteoclast which causes osteoporosis.

To overcome the problem, the researchers have developed RANKL fragment peptides which block the activation of microglia, macrophage, and osteoclast [2]. Since the peptides can attenuate inflammatory immune response, and competitively inhibits RANKL/RANK pathway of osteoclast, they can be an alternative of TLR2/4 antagonists or RANKL antagonists, and possibly applicable for sepsis and osteoporosis.

[1] Proc Natl Acad Sci U S A. 2014 Jun 3; 111(22): 8191-6. doi: 10.1073/pnas.1400544111.
[2] Scientific Reports 6, Article number: 38062 (2016) doi:10.1038/srep38062

Facts and Data

  1. The invented 30-amino acid peptide significantly reduced secretion of IL-6, TNF-alpha from LPS stimulated mouse/human macrophage, mouse microglia cell lines, and prevented neuronal death of LPS stimulated neuron/glia mixed primary culture.
  2. The peptide inhibited RNANK/RANKL dependent M1 macrophage differentiation and osteoclast progenitor cell maturation.
  3. In mouse ischemic stroke/reperfusion model, i administration after 6 hours of onset of stroke successfully delivered the peptide through the blood brain barrier, reduced infarct volume to less than a half(n=7) and NSS(neurological severity score) from around 2 to 1.

NOTE: The peptide used was not protected or modified for protease degradation avoidance.

Patent/Publications

Patent US,CA,EP,AU,CN,JP

Researcher

Associate Prof.  Munehisa Shimamura, Graduate School of Medicine, Osaka University, Japan

http://www.dma.jim.osaka-u.ac.jp/view?l=en&u=6516

Expectations

We are seeking a licensee. Details can be disclosed under CDA.

Product No: TP0521

Inquiry Form

    Your name (*required)

    E-mail address (*required)

    Company name

    Message (*required)

    Following submission of your inquiry

    We will contact you shortly to discuss confidentiality, materials transfer, evaluation steps, and licensing opportunities.

    <Notice>

    Our support is provided free of charge.
    The information submitted on this form is for business development use only.

    By clicking "Send", you are agreeing to our Privacy Policy.
    If you have questions please reach out to info (at) tech-manage.co.jp.

    About Bionauts.jp Tech Manage Corp.
    Copyright © Tech Manage Corp. All Rights Reserved.
    This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.