Advantage and Core Benefit
- Natural proteins easily functionalized.
- Homologous molecule obtained with no sidechain reaction.
- No limitation of N-terminal amino acid residue, except proline.
Potential Applications
- Pharmaceuticals: Antigen-drug conjugates (ADCs), PEGylation, New modalities (e.g. DNA aptamer-antibody Fc conjugates), TR-FRET libraries for drug discovery, Radio-active labeling for pharmacokinetics
- Diagnostics: ELISA/RIA, PET/SPECT
Background and Technology
There are several protein linking methods for functionalization. Some methods non-specifically modify functional groups of protein, which might affect original function of the protein, whereas others need protein modification at a gene design level.
Newly developed linker 1H-1,2,3-triazole-4-carbaldehyde (TA4C) derivatives selectively react with N-terminal amine of proteins, which enables to efficiently introduce new functional groups without modifying original function/structure of protein.
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Data
- TA4C-ligands are easily synthesized with 1-3 steps using previously reported methods.
- – 80% of RNase was conjugated with Bn using TA4C-Bn within 16 hrs. at pH7.5 and 37℃
Patent/Publication
- PCT/JP2020/008357; US, EP, CN, J
- https://doi.org/10.1002/cbic.201900692
Researchers
Prof. Akira Onoda (Hokkaido University)
Expectations
- We are seeking companies to license and commercialize this technology.
- Samples for your evaluation are available under material transfer agreement (MTA).
Project No: KJ-02501