- Functionalized natural proteins are easily available
- Homologous molecules can be obtained with high efficiency and no sidechain reaction.
- No restriction of N-terminal amino acid species
- Pharmaceuticals: Antigen-drug conjugates (ADCs), PEGylation, New modalities (e.g. DNA aptamer-antibody Fc conjugates), TR-FRET libraries for drug discovery, Radio-active labeling for pharmacokinetics
- Diagnostics: ELISA/RIA, PET/SPECT
Background and Technology
There are several protein linking methods for functionalization. Some methods non-specifically modify functional groups of protein, which might affect original function of the protein, whereas others need protein modification at a gene design level.
Newly developed two linkers selectively react with N-terminal amine and enable to efficiently introduce new functional groups without modifying original function/structure of protein.
Linker 1 (TA4C method)
- TA4C-ligands are synthesized easily by 1-3 steps using previously reported methods.
- ～80% of RNase was conjugated with Bn using TA4C-Bn within 16 hrs at pH7.5 and 37℃
Linker 2 (6AMPC method)
- 6AMPC is synthesized by 5 steps.
- ~80% of RNase was modified with 6AMPC at pH7.5 and 37℃ for 16 hrs, followed by conjugation with a ligand compound through CuAAC reaction at pH7.5 and room temperature for~2hrs.
Prof. Takashi HAYASHI Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Japan (http://www.applied-bioinorganic.jp/en/).
- We are seeking companies to license and commercialize this technology.
- Samples for your evaluation are available under material transfer agreement (MTA).
Product No. : CD-02501