{"id":991,"date":"2017-11-17T09:54:40","date_gmt":"2017-11-17T00:54:40","guid":{"rendered":"https:\/\/bionauts.jp\/?p=991"},"modified":"2025-03-17T13:03:28","modified_gmt":"2025-03-17T04:03:28","slug":"cellular-cytotoxicity-assay-system","status":"publish","type":"post","link":"https:\/\/bionauts.jp\/?p=991","title":{"rendered":"Cellular Cytotoxicity Assay System for Immuno-Oncology"},"content":{"rendered":"<h3>Application<\/h3>\n<p>Time-resolved fluorescene (TRF) reagent for assays of the cytotoxic activity of immune effector cells against tumor cells (including ones which are opsonized with antibodies).<\/p>\n<h3>Advantages<\/h3>\n<ul>\n<li>Non-RI small molecule<\/li>\n<li>Lower background level<\/li>\n<li>Shorter measurement time<\/li>\n<\/ul>\n<h3>Technology Overview &amp; Background<\/h3>\n<p>A novel non-RI reagent for TRF with highly maximum release and lower spontaneous release from cell.\u00a0 This molecule is more potent than well-known chelating lanthanide-labeled TRF reagent.<\/p>\n<p>When the tumor cells are pretreated with this novel pro-chelate-forming compound, its hydrolyzate is released upon disruption of the tumor cell membrane during the cellular killing.\u00a0 Upon addition of Europium (Eu), the cellular cytotoxicity can be determined by TRF within very short measuring time (30\u201340 min.).<\/p>\n<p><a href=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2017\/11\/Fig0.png\"><img decoding=\"async\" loading=\"lazy\" class=\"size-large wp-image-4313 aligncenter\" src=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2017\/11\/Fig0-1024x354.png\" alt=\"\" width=\"840\" height=\"290\" srcset=\"https:\/\/bionauts.jp\/wp-content\/uploads\/2017\/11\/Fig0-1024x354.png 1024w, https:\/\/bionauts.jp\/wp-content\/uploads\/2017\/11\/Fig0-300x104.png 300w, https:\/\/bionauts.jp\/wp-content\/uploads\/2017\/11\/Fig0-768x266.png 768w, https:\/\/bionauts.jp\/wp-content\/uploads\/2017\/11\/Fig0-1536x531.png 1536w, https:\/\/bionauts.jp\/wp-content\/uploads\/2017\/11\/Fig0-1200x415.png 1200w, https:\/\/bionauts.jp\/wp-content\/uploads\/2017\/11\/Fig0.png 1610w\" sizes=\"(max-width: 709px) 85vw, (max-width: 909px) 67vw, (max-width: 1362px) 62vw, 840px\" \/><\/a><a href=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2017\/11\/Fig1.png\"><img decoding=\"async\" loading=\"lazy\" class=\"size-full wp-image-4249 aligncenter\" src=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2017\/11\/Fig1.png\" alt=\"\" width=\"528\" height=\"236\" srcset=\"https:\/\/bionauts.jp\/wp-content\/uploads\/2017\/11\/Fig1.png 528w, https:\/\/bionauts.jp\/wp-content\/uploads\/2017\/11\/Fig1-300x134.png 300w\" sizes=\"(max-width: 528px) 85vw, 528px\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<h3>Publications<\/h3>\n<p>Sakai Y., <span style=\"text-decoration: underline;\">Tanaka Y.<\/span>, <em>et al<\/em>. \u201cLive Cell Labeling with Terpyridine Derivative Proligands to Measure Cytotoxicity Mediated by Immune Cells.\u201d<br \/>\n<em>ChemMedChem<\/em> 2017; 12(23): 2006\u20132013.<br \/>\n[DOI] <a href=\"https:\/\/doi.org\/10.1002\/cmdc.201700626\" target=\"_blank\" rel=\"noopener noreferrer\">10.1002\/cmdc.201700626<\/a>.<\/p>\n<h3>Patents<\/h3>\n<p>Issued in Japan (JP 6501271), Europe (EP 3127899) and the United States (US 10175229).<\/p>\n<h3>Principal Investigator<\/h3>\n<p>Yoshimasa TANAKA, PhD (Professor, Nagasaki University, Japan)<\/p>\n<h3>Expectations<\/h3>\n<p><strong>TECH MANAGE <\/strong>is looking for both biotech companies to develop this technology and sell products for research use, and pharmaceutical companies and CDMOs to use for the evaluation for their clinical purposes (i.e. for cell therapy-related products).<\/p>\n<p>&nbsp;<\/p>\n<p>Project ID: JT-00546<\/p>\n","protected":false},"excerpt":{"rendered":"<p>By using a novel, non-radioactive, chelate-forming compound.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0},"categories":[1],"tags":[],"categories_of_inventions":[48],"disease_categories___indication":[61],"asset_type":[74,78,87],"development_phase":[],"medicine":[109],"other_than_medicine":[134,130],"material":[],"engineering":[],"software_it":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/991"}],"collection":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=991"}],"version-history":[{"count":14,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/991\/revisions"}],"predecessor-version":[{"id":4314,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/991\/revisions\/4314"}],"wp:attachment":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=991"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=991"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=991"},{"taxonomy":"categories_of_inventions","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories_of_inventions&post=991"},{"taxonomy":"disease_categories___indication","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fdisease_categories___indication&post=991"},{"taxonomy":"asset_type","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fasset_type&post=991"},{"taxonomy":"development_phase","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fdevelopment_phase&post=991"},{"taxonomy":"medicine","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedicine&post=991"},{"taxonomy":"other_than_medicine","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fother_than_medicine&post=991"},{"taxonomy":"material","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmaterial&post=991"},{"taxonomy":"engineering","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fengineering&post=991"},{"taxonomy":"software_it","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fsoftware_it&post=991"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}