{"id":4900,"date":"2025-10-17T19:15:09","date_gmt":"2025-10-17T10:15:09","guid":{"rendered":"https:\/\/bionauts.jp\/?p=4900"},"modified":"2025-10-17T19:17:05","modified_gmt":"2025-10-17T10:17:05","slug":"highly-efficient-trap-display","status":"publish","type":"post","link":"https:\/\/bionauts.jp\/?p=4900","title":{"rendered":"Highly Efficient TRAP Display"},"content":{"rendered":"<h3>Advantages<\/h3>\n<ul>\n<li>Improved display efficiency and post-transcriptional diversity<\/li>\n<li>Versatile and independent of functional molecules<\/li>\n<li>Established method for selecting specific RNA sequences<\/li>\n<li>Applicable to other RNA display methods<\/li>\n<\/ul>\n<table style=\"border-collapse: collapse; width: 74%; height: 408px;\">\n<tbody>\n<tr>\n<td style=\"width: 100%;\"><a href=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2025\/10\/BK-03990_upper_EN-1.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignleft wp-image-4902 size-large\" src=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2025\/10\/BK-03990_upper_EN-1-1024x868.png\" alt=\"\" width=\"840\" height=\"712\" srcset=\"https:\/\/bionauts.jp\/wp-content\/uploads\/2025\/10\/BK-03990_upper_EN-1-1024x868.png 1024w, https:\/\/bionauts.jp\/wp-content\/uploads\/2025\/10\/BK-03990_upper_EN-1-300x254.png 300w, https:\/\/bionauts.jp\/wp-content\/uploads\/2025\/10\/BK-03990_upper_EN-1-768x651.png 768w, https:\/\/bionauts.jp\/wp-content\/uploads\/2025\/10\/BK-03990_upper_EN-1-1200x1017.png 1200w, https:\/\/bionauts.jp\/wp-content\/uploads\/2025\/10\/BK-03990_upper_EN-1.png 1534w\" sizes=\"(max-width: 709px) 85vw, (max-width: 909px) 67vw, (max-width: 1362px) 62vw, 840px\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>Current Stage and Key Data<\/h3>\n<ul>\n<li>The effectiveness of TRAP display has been confirmed using monobodies and cyclic peptides as functional molecule candidates.<\/li>\n<li>Improve display efficiency by a few percent to around 20%.<\/li>\n<\/ul>\n<table style=\"border-collapse: collapse; width: 100%;\">\n<tbody>\n<tr>\n<td style=\"width: 100%;\"><a href=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2025\/10\/BK-03990_lower_EN.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignleft size-full wp-image-4903\" src=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2025\/10\/BK-03990_lower_EN.png\" alt=\"\" width=\"665\" height=\"277\" srcset=\"https:\/\/bionauts.jp\/wp-content\/uploads\/2025\/10\/BK-03990_lower_EN.png 665w, https:\/\/bionauts.jp\/wp-content\/uploads\/2025\/10\/BK-03990_lower_EN-300x125.png 300w\" sizes=\"(max-width: 709px) 85vw, (max-width: 909px) 67vw, (max-width: 984px) 61vw, (max-width: 1362px) 45vw, 600px\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>Partnering Model<\/h3>\n<p>License this technology to companies using TRAP or other RNA display technologies.<\/p>\n<ul>\n<li>Potential partners: Pharmaceutical\/biotech companies and CROs focusing on the discovery\/development of peptide, medium molecule, and antibody drugs<\/li>\n<\/ul>\n<h3>Background<\/h3>\n<p>Functional molecules (peptides and proteins) with high affinity for a target are candidates for detection tools and therapeutic drugs. RNA display methods, including the TLAP method, are efficient screening methods for functional molecules by displaying phenotypes of functional molecules on mRNA (genetic type) via puromycin linker ( PuL ) based on functional molecule DNA libraries. Therefore, it is important to ensure the diversity of functional molecule-displaying mRNA based on the diversity of the DNA library.<br \/>\nTo ensure diversity in TRAP display, it is necessary to improve the display efficiency. Three factors determine the display efficiency: the binding efficiency of mRNA and PuL , the translation efficiency of mRNA to protein, and the ligation efficiency of protein and PuL. We worked to improve this efficiency.<\/p>\n<h3>Patents and Publications<\/h3>\n<ul>\n<li><a href=\"https:\/\/doi.org\/10.1093\/nar\/gkad555\" target=\"_blank\" rel=\"noopener\">Umemoto et al., Nucleic Acids Research (2023) Volume 51, Issue 14, P. 7465\u20137479<\/a><\/li>\n<li>Japan: Patent registered (No. 7654312), USA: Patent pending (Pub No. US2025017770A1)<\/li>\n<\/ul>\n<h3>Principal Investigator<\/h3>\n<p>Hiroshi Murakami (Graduate School of Engineering, Nagoya University, Tokai National Higher Education and Research System)<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>Project ID:BK-03990<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A specific RNA sequence added to the 3&#8242; end of the mRNA sequence of a functional molecule candidate significantly improves the display efficiency of the TRAP method and ensures diversity after transcription and translation.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0},"categories":[1],"tags":[],"categories_of_inventions":[48],"disease_categories___indication":[],"asset_type":[],"development_phase":[],"medicine":[],"other_than_medicine":[],"material":[],"engineering":[],"software_it":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/4900"}],"collection":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4900"}],"version-history":[{"count":2,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/4900\/revisions"}],"predecessor-version":[{"id":4905,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/4900\/revisions\/4905"}],"wp:attachment":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4900"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4900"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4900"},{"taxonomy":"categories_of_inventions","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories_of_inventions&post=4900"},{"taxonomy":"disease_categories___indication","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fdisease_categories___indication&post=4900"},{"taxonomy":"asset_type","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fasset_type&post=4900"},{"taxonomy":"development_phase","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fdevelopment_phase&post=4900"},{"taxonomy":"medicine","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedicine&post=4900"},{"taxonomy":"other_than_medicine","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fother_than_medicine&post=4900"},{"taxonomy":"material","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmaterial&post=4900"},{"taxonomy":"engineering","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fengineering&post=4900"},{"taxonomy":"software_it","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fsoftware_it&post=4900"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}