{"id":4524,"date":"2025-05-14T11:24:22","date_gmt":"2025-05-14T02:24:22","guid":{"rendered":"https:\/\/bionauts.jp\/?p=4524"},"modified":"2025-05-16T21:32:45","modified_gmt":"2025-05-16T12:32:45","slug":"urinary-biomarker-for-aging-assessment","status":"publish","type":"post","link":"https:\/\/bionauts.jp\/?p=4524","title":{"rendered":"Urinary Biomarker for Aging Assessment"},"content":{"rendered":"<h3>Advantage and Core Benefit<\/h3>\n<ul>\n<li>Non-invasive Aging Diagnosis: L-Saccharopine measurement in blood or urine enables a simple and convenient assessment of aging progression.<\/li>\n<li>Validity as a Biomarker: Strong correlation with progeroid syndromes and mitochondrial dysfunction supports its reliability as an aging biomarker.<\/li>\n<li>Insight into Anti-Aging Effects: Findings suggest that restricting lysine metabolism has anti-aging effects, potentially leading to the development of anti-aging drugs and functional foods.<\/li>\n<\/ul>\n<h3>Background and Technology<\/h3>\n<p>Traditional aging biomarkers include senescence-associated \u03b2-galactosidase activity, telomere shortening, and cell cycle inhibitors (p21, p16, etc.). However, these assessments require cell or tissue sampling and analysis, making them invasive. Thus, there is a demand for simpler biomarkers that can be measured using blood or urine samples. L-Saccharopine is an intermediate metabolite in mitochondrial lysine degradation. When it accumulates in mitochondria, it causes a decrease in membrane potential and an increase in reactive oxygen species (ROS), leading to mitochondrial dysfunction and toxicity (<a href=\"https:\/\/doi.org\/10.1083\/jcb.201807204\" target=\"_blank\" rel=\"noopener\">DOI: 10.1083\/jcb.201807204<\/a>).<\/p>\n<p>The inventors conducted metabolomic analysis using cells derived from Werner syndrome (WS) patients, a premature aging disease, and discovered that intracellular L-saccharopine levels were significantly elevated in these cells. Further measurement of urinary and plasma L-saccharopine concentrations in both healthy individuals and WS patients revealed that WS patients had significantly higher L-saccharopine levels than healthy controls. Additionally, in healthy individuals, L-saccharopine levels increased with age.<\/p>\n<p>These results indicate that urinary L-saccharopine concentration increases with aging, functioning as both an aging biomarker and a mitochondrial dysfunction-induced aging toxin. Unlike conventional cell- and tissue-based aging assessments, urine samples can be collected non-invasively, making L-saccharopine a practical and user-friendly biomarker for aging assessment.<\/p>\n<h3>Data<\/h3>\n<ul>\n<li>L-Saccharopine was quantified using HPLC in urine samples from 29 healthy individuals (ages 22\u201389) and 10 WS patients (ages 45\u201366), normalized to urinary creatinine (U-Cre). WS patients had significantly higher urinary L-saccharopine levels than healthy controls. In healthy individuals, urinary L-saccharopine levels tended to increase with age.<\/li>\n<li>A threshold of 671.9 \u00b5g\/g U-Cr for identifying individuals aged 70 and older yielded a specificity of 80% and a sensitivity of 84.2%.<\/li>\n<li>WS patient-derived cells were cultured for four months in lysine-containing and lysine-restricted media. Under lysine restriction, cell proliferation (cPDL) was maintained for two months, and the proportion of senescent cells (assessed via \u03b2-galactosidase staining) was significantly reduced.<\/li>\n<\/ul>\n<table style=\"border-collapse: collapse; width: 77.6667%; height: 500px;\">\n<tbody>\n<tr>\n<td style=\"width: 100%;\"><a href=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2025\/05\/WL-05160.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignleft size-full wp-image-4525\" src=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2025\/05\/WL-05160.png\" alt=\"\" width=\"780\" height=\"802\" srcset=\"https:\/\/bionauts.jp\/wp-content\/uploads\/2025\/05\/WL-05160.png 780w, https:\/\/bionauts.jp\/wp-content\/uploads\/2025\/05\/WL-05160-292x300.png 292w, https:\/\/bionauts.jp\/wp-content\/uploads\/2025\/05\/WL-05160-768x790.png 768w\" sizes=\"(max-width: 709px) 85vw, (max-width: 909px) 67vw, (max-width: 984px) 61vw, (max-width: 1362px) 45vw, 600px\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>Patent<\/h3>\n<p>WO2022259950<\/p>\n<h3>Researcher<\/h3>\n<p>Dr. Naoya Kato, Dr. Yasuo Ouchi, Dr. Hiyori Kaneko, Dr. Zenro Maezawa, Dr. Hiroyuki Eto, Dr. Kotaro Yokote (Chiba University, Faculty of Medicine)<\/p>\n<h3>Expectations<\/h3>\n<p>We seek partnerships with companies interested in drug discovery and anti-aging product development through collaborative research on aging biomarkers and mitochondrial metabolism.<br \/>\nPotential collaboration partners include:<br \/>\nDiagnostic and chemical companies: Developing simple and efficient L-saccharopine measurement methods.<br \/>\nPharmaceutical companies: Evaluating anti-aging drugs and compounds affecting mitochondrial function using biological samples.<br \/>\nHealth food and anti-aging product developers: Assessing product efficacy through urinary L-saccharopine measurements.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>Project ID:WL-05160<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>L-Saccharopine: A Metabolic Marker Reflecting Mitochondrial Dysfunction as an Indicator of Aging<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0},"categories":[1],"tags":[],"categories_of_inventions":[47],"disease_categories___indication":[],"asset_type":[],"development_phase":[],"medicine":[],"other_than_medicine":[127,128],"material":[],"engineering":[],"software_it":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/4524"}],"collection":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4524"}],"version-history":[{"count":3,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/4524\/revisions"}],"predecessor-version":[{"id":4528,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/4524\/revisions\/4528"}],"wp:attachment":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4524"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4524"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4524"},{"taxonomy":"categories_of_inventions","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories_of_inventions&post=4524"},{"taxonomy":"disease_categories___indication","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fdisease_categories___indication&post=4524"},{"taxonomy":"asset_type","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fasset_type&post=4524"},{"taxonomy":"development_phase","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fdevelopment_phase&post=4524"},{"taxonomy":"medicine","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedicine&post=4524"},{"taxonomy":"other_than_medicine","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fother_than_medicine&post=4524"},{"taxonomy":"material","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmaterial&post=4524"},{"taxonomy":"engineering","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fengineering&post=4524"},{"taxonomy":"software_it","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fsoftware_it&post=4524"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}