{"id":3648,"date":"2024-05-13T17:24:30","date_gmt":"2024-05-13T08:24:30","guid":{"rendered":"https:\/\/bionauts.jp\/?p=3648"},"modified":"2024-05-19T23:38:20","modified_gmt":"2024-05-19T14:38:20","slug":"high-protein-expression-and-production-system-in-plants","status":"publish","type":"post","link":"https:\/\/bionauts.jp\/?p=3648","title":{"rendered":"High Protein Expression and Production System in Plants"},"content":{"rendered":"<h3>Advantage and Core Benefit<\/h3>\n<ul>\n<li>Obtains desired protein faster than conventional techniques such as magnICON<\/li>\n<li>Protein expression is increased by suppressing necrosis by spraying with ascorbic acid.<\/li>\n<li>Applicable to <em>Solanaceae, Asteraceae, Cucurbitaceae<\/em>, and <em>Leguminosae<\/em>, and expected to be applied to genome editing and production of useful metabolites.<\/li>\n<\/ul>\n<h3>Background and Technology<\/h3>\n<table style=\"border-collapse: collapse; width: 63.6667%; height: 164px;\">\n<tbody>\n<tr style=\"height: 206px;\">\n<td style=\"width: 100%; height: 206px;\"><a href=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2024\/05\/WL-04918a.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignleft wp-image-3649\" src=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2024\/05\/WL-04918a.png\" alt=\"\" width=\"348\" height=\"103\" srcset=\"https:\/\/bionauts.jp\/wp-content\/uploads\/2024\/05\/WL-04918a.png 970w, https:\/\/bionauts.jp\/wp-content\/uploads\/2024\/05\/WL-04918a-300x89.png 300w, https:\/\/bionauts.jp\/wp-content\/uploads\/2024\/05\/WL-04918a-768x227.png 768w\" sizes=\"(max-width: 348px) 85vw, 348px\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Protein production in plants is expected to be advantageous in terms of running cost, but one of the challenges is that the expression level is lower than that of <em>E. coli<\/em> etc.<br \/>\nAs a method of protein production in plants, the agroinfiltration method has been developed to transiently express target proteins by <em>Agrobacterium<\/em> infection. The amount of protein expression in the agroinfiltration method depends on the vector that carries the gene from the bacterium to the plant cell.<br \/>\nThe inventors have succeeded in expressing large amounts of protein by modifying the vector in a system that infects<em> Nicotiana benthamiana<\/em>. By incorporating a Geminivirus replication system in the vector and including a double terminator, the mRNA encoding the target gene was transcribed in large quantities, resulting in an expression level of approximately 4 mg of protein per gram fresh weight (FW). This amount of protein expression is comparable to that of expression systems such as <em>E. coli<\/em>. Compared to magnICON, an existing plant expression system, it has an advantage in terms of rapid production.<br \/>\nIn addition, protein expression is now possible not only in <em>N. benthamiana <\/em>but also in various other plants such as lettuce, eggplant, tomato, soybean, and melon, and can be used for genome editing in each of these plants.<\/p>\n<table style=\"border-collapse: collapse; width: 100%;\">\n<tbody>\n<tr>\n<td style=\"width: 100%;\"><a href=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2024\/05\/WL-04918b.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignleft size-full wp-image-3650\" src=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2024\/05\/WL-04918b.png\" alt=\"\" width=\"1003\" height=\"175\" srcset=\"https:\/\/bionauts.jp\/wp-content\/uploads\/2024\/05\/WL-04918b.png 1003w, https:\/\/bionauts.jp\/wp-content\/uploads\/2024\/05\/WL-04918b-300x52.png 300w, https:\/\/bionauts.jp\/wp-content\/uploads\/2024\/05\/WL-04918b-768x134.png 768w\" sizes=\"(max-width: 709px) 85vw, (max-width: 909px) 67vw, (max-width: 1362px) 62vw, 840px\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>Examples of Application<\/h3>\n<ol>\n<li>Expression of proteins and complexes that are difficult to express in <em> coli <\/em>and other bacteria<br \/>\nHuman Cullin protein, antibody formation (<a href=\"https:\/\/doi.org\/10.5511\/plantbiotechnology.21.0610a\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.5511\/plantbiotechnology.21.0610a<\/a>)<\/li>\n<li>Production of pharmaceutical-related proteins<br \/>\nBirch pollen allergen Bet v 1 binding to IgE in allergic patients (<a href=\"https:\/\/doi.org\/10.3389\/fpls.2020.00344\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.3389\/fpls.2020.00344<\/a>)<br \/>\nRecombinant lactoferrin production (<a href=\"https:\/\/doi.org\/10.5511\/plantbiotechnology.23.0128a\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.5511\/plantbiotechnology.23.0128a<\/a>)<\/li>\n<li>Substance production by biosynthetic enzyme expression<br \/>\n4DO (4-deoxyrobanchol) accumulation (2.1 mg\/g FW) by expression of large amounts of metabolic enzymes; D27, CCD7, CCD8, CYP711A2. (<a href=\"https:\/\/doi.org\/10.3389\/fpls.2022.1027004\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.3389\/fpls.2022.1027004<\/a>)<\/li>\n<\/ol>\n<h3>Patent<\/h3>\n<p>Basic patent: JP6850041B, CA3059025C (Granted) US2021-0108218, EP3604546(Pending)<br \/>\nImproved invention: CA3144718C (Granted), JP2021-020421, US2022-0256847, EP4008723(Pending)<br \/>\nApplication patent (genome editing): Pending in Japan<\/p>\n<h3>Researcher<\/h3>\n<p>Dr. Kenji Miura (University of Tsukuba)<\/p>\n<h3>Expectations<\/h3>\n<p>We are looking for companies that are interested in licensing the patents related to our invention, a system for high protein production in plants, to work on protein production in plants, production of secondary metaboloites, and generation of genome-edited crops. Licenses for research and development purposes are also available. We can also provide a paid MTA of the vector (pBYR2HS, pTKB3) for evaluation studies.<\/p>\n<p>&nbsp;<\/p>\n<p>Project.WL-04918<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tsukuba System: World&#8217;s strongest vector for transient protein expression with a yield of 4 mg\/g<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0},"categories":[1],"tags":[],"categories_of_inventions":[49],"disease_categories___indication":[],"asset_type":[],"development_phase":[],"medicine":[],"other_than_medicine":[143,141],"material":[],"engineering":[],"software_it":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/3648"}],"collection":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3648"}],"version-history":[{"count":5,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/3648\/revisions"}],"predecessor-version":[{"id":3656,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/3648\/revisions\/3656"}],"wp:attachment":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3648"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3648"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3648"},{"taxonomy":"categories_of_inventions","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories_of_inventions&post=3648"},{"taxonomy":"disease_categories___indication","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fdisease_categories___indication&post=3648"},{"taxonomy":"asset_type","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fasset_type&post=3648"},{"taxonomy":"development_phase","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fdevelopment_phase&post=3648"},{"taxonomy":"medicine","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedicine&post=3648"},{"taxonomy":"other_than_medicine","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fother_than_medicine&post=3648"},{"taxonomy":"material","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmaterial&post=3648"},{"taxonomy":"engineering","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fengineering&post=3648"},{"taxonomy":"software_it","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fsoftware_it&post=3648"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}