{"id":3467,"date":"2024-03-07T15:14:29","date_gmt":"2024-03-07T06:14:29","guid":{"rendered":"https:\/\/bionauts.jp\/?p=3467"},"modified":"2025-12-16T22:19:19","modified_gmt":"2025-12-16T13:19:19","slug":"myeloid-cells-with-proliferation-ability-from-human-peripheral-blood-monocytes-or-pluripotent-stem-cells-pscs-2","status":"publish","type":"post","link":"https:\/\/bionauts.jp\/?p=3467","title":{"rendered":"Myeloid Cell Line with proliferation ability  from human iPSCs or Monocyte"},"content":{"rendered":"<h3>Advantages<\/h3>\n<ul>\n<li>Available for large scale of dendritic cells (DC) or macrophages (MP)<\/li>\n<li>Long-prolonged proliferation period; minimum period is 3 months<\/li>\n<li>Dendritic cells derived from Myeloid Cell Line could be model cells for virus research<\/li>\n<li>Further established interferon-\u03b2\/interferon-\u03b3 expressing iPS-ML for cell therapy<\/li>\n<\/ul>\n<h3>Background &amp; Technology<\/h3>\n<p>Myeloid blood cells are a group of cells classified within leukocytes and include macrophages, dendritic cells, and granulocytes. Macrophages and dendritic cells are used as cell therapy, and to achieve clinical efficacy, large numbers of cells are needed to achieve clinical efficacy. Until now, no method has been reported that can sustain proliferation for a long period of time (more than one month) and produce large numbers of myeloid blood cells, because it required considerable labor and time.<br \/>\nThis technology is used to induce the forcible expression of various genes in ML derived from pluripotent stem cells (iPSCs) or peripheral blood monocytes, thereby adding the ability for long-term proliferation. Specifically, this is a method for producing ML from iPSCs (iPS-ML) or Monocytes (Monocytes-ML) with proliferation capability which includes the forcible expression of cMYC and the other exogenous gene.<br \/>\nInventor established a method of xeno-free differentiation culture to generate a large scale of functional dendritic cells (iPS-DC) \/macrophages (iPS-MP), Monocytes-DC\/macrophages (Monocytes-MP) and demonstrated their potential for cell-therapy, drug screening or research application.<\/p>\n<table style=\"border-collapse: collapse; width: 59.3333%; height: 252px;\">\n<tbody>\n<tr>\n<td style=\"width: 100%;\"><a href=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2016\/09\/ON-00721.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignleft size-large wp-image-3466\" src=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2016\/09\/ON-00721-1024x612.png\" alt=\"\" width=\"840\" height=\"502\" srcset=\"https:\/\/bionauts.jp\/wp-content\/uploads\/2016\/09\/ON-00721-1024x612.png 1024w, https:\/\/bionauts.jp\/wp-content\/uploads\/2016\/09\/ON-00721-300x179.png 300w, https:\/\/bionauts.jp\/wp-content\/uploads\/2016\/09\/ON-00721-768x459.png 768w, https:\/\/bionauts.jp\/wp-content\/uploads\/2016\/09\/ON-00721-1536x918.png 1536w, https:\/\/bionauts.jp\/wp-content\/uploads\/2016\/09\/ON-00721-2048x1224.png 2048w, https:\/\/bionauts.jp\/wp-content\/uploads\/2016\/09\/ON-00721-1200x717.png 1200w\" sizes=\"(max-width: 709px) 85vw, (max-width: 909px) 67vw, (max-width: 1362px) 62vw, 840px\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: center;\">\u00a0 <strong>Outline of method of producing Myeloid blood cell lines<\/strong><\/p>\n<h3>Data<\/h3>\n<p>Proliferative myeloid cells derived from iPS cells (iPS-ML)<\/p>\n<table style=\"border-collapse: collapse; width: 63.6666%; height: 308px;\">\n<tbody>\n<tr style=\"height: 360px;\">\n<td style=\"width: 100%; height: 360px;\"><a href=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2024\/03\/ON-00721b.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignleft size-large wp-image-3469\" src=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2024\/03\/ON-00721b-1024x572.png\" alt=\"\" width=\"840\" height=\"469\" srcset=\"https:\/\/bionauts.jp\/wp-content\/uploads\/2024\/03\/ON-00721b-1024x572.png 1024w, https:\/\/bionauts.jp\/wp-content\/uploads\/2024\/03\/ON-00721b-300x168.png 300w, https:\/\/bionauts.jp\/wp-content\/uploads\/2024\/03\/ON-00721b-768x429.png 768w, https:\/\/bionauts.jp\/wp-content\/uploads\/2024\/03\/ON-00721b-1536x858.png 1536w, https:\/\/bionauts.jp\/wp-content\/uploads\/2024\/03\/ON-00721b-2048x1144.png 2048w, https:\/\/bionauts.jp\/wp-content\/uploads\/2024\/03\/ON-00721b-1200x670.png 1200w\" sizes=\"(max-width: 709px) 85vw, (max-width: 909px) 67vw, (max-width: 1362px) 62vw, 840px\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: center;\"><span style=\"font-size: 10pt;\"><strong>Morphology of iPS-ML derived dendritic cell like cells generated by forced expression of cMYC and BMI1<\/strong><\/span><\/p>\n<h3>Patent<\/h3>\n<p>JP5861191, US10,034,900<\/p>\n<h3>Publication<\/h3>\n<p>[1] Haruta et l. (2013) <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22875043\" target=\"_blank\" rel=\"noopener\">Gene Ther.<\/a>\u00a0May;20(5):504-13.<br \/>\n[2] Haruta et al. (2013) Hum Immnol.;74(10):1400-8.<br \/>\n[3] Koba et al. (2013) PLos One ;8(6): e67567<br \/>\n[4] Dao Huy Manh et al. (2018) <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30058991\" target=\"_blank\" rel=\"noopener\">J Gen Virol.<\/a>;2018 Sep;99(9):1239-1247<\/p>\n<h3>Applicant<\/h3>\n<p>National University Kumamoto University<\/p>\n<h3>Expectation<\/h3>\n<p>We are looking for partner companies interested in license-in and utilizing this method and technology. We would be happy to start with a detailed explanation and discussion of the technology.<\/p>\n<p>Project No. ON-00721<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Advantages Available for large scale of dendritic cells (DC) or macrophages (MP) Long-prolonged proliferation  &hellip; <a href=\"https:\/\/bionauts.jp\/?p=3467\" class=\"more-link\"><span class=\"screen-reader-text\">&#8220;Myeloid Cell Line with proliferation ability  from human iPSCs or Monocyte&#8221; \u306e<\/span>\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0},"categories":[1],"tags":[],"categories_of_inventions":[48],"disease_categories___indication":[],"asset_type":[],"development_phase":[],"medicine":[109,110],"other_than_medicine":[140,138],"material":[],"engineering":[],"software_it":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/3467"}],"collection":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3467"}],"version-history":[{"count":8,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/3467\/revisions"}],"predecessor-version":[{"id":5006,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/3467\/revisions\/5006"}],"wp:attachment":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3467"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3467"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3467"},{"taxonomy":"categories_of_inventions","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories_of_inventions&post=3467"},{"taxonomy":"disease_categories___indication","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fdisease_categories___indication&post=3467"},{"taxonomy":"asset_type","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fasset_type&post=3467"},{"taxonomy":"development_phase","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fdevelopment_phase&post=3467"},{"taxonomy":"medicine","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedicine&post=3467"},{"taxonomy":"other_than_medicine","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fother_than_medicine&post=3467"},{"taxonomy":"material","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmaterial&post=3467"},{"taxonomy":"engineering","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fengineering&post=3467"},{"taxonomy":"software_it","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fsoftware_it&post=3467"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}