{"id":1286,"date":"2019-05-15T12:34:29","date_gmt":"2019-05-15T03:34:29","guid":{"rendered":"https:\/\/bionauts.jp\/?p=1286"},"modified":"2025-03-18T16:10:04","modified_gmt":"2025-03-18T07:10:04","slug":"highly-specificefficient-protein-linker","status":"publish","type":"post","link":"https:\/\/bionauts.jp\/?p=1286","title":{"rendered":"Highly Specific\/Efficient Protein Linker"},"content":{"rendered":"<h3>Advantage and Core Benefit<\/h3>\n<ul>\n<li>Natural proteins are easily functionalized.<\/li>\n<li>Homogeneous proteins site-specifically liked with a molecule are obtained without any reaction on sidechain.<\/li>\n<li>No limitation of N-terminal amino acid residue.<\/li>\n<\/ul>\n<h3>Potential Applications<\/h3>\n<ul>\n<li>Pharmaceuticals: Antibody-drug conjugates (ADCs), PEGylation, New modalities (e.g. DNA aptamer-antibody Fc conjugates), TR-FRET libraries for drug discovery, Radio-active labeling for pharmacokinetics<\/li>\n<li>Diagnostics: ELISA\/RIA, PET\/SPECT<\/li>\n<\/ul>\n<h3>Background and Technology<\/h3>\n<p>There are several protein linking methods for functionalization. Some methods non-specifically modify functional groups of protein, which might affect original function of the protein, whereas others need protein modification at a gene design level.<br \/>\nNewly developed linker 1<em>H<\/em>-1,2,3-triazole-4-carbaldehyde (TA4C) derivatives selectively react with N-terminal amine of proteins, which enable to efficiently introduce new functional groups without modifying original function\/structure of protein (fig.L).<\/p>\n<table style=\"border-collapse: collapse; width: 100%;\">\n<tbody>\n<tr>\n<td style=\"width: 100%;\"><a href=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2019\/05\/KJ-02501.png\"><img decoding=\"async\" loading=\"lazy\" class=\"alignleft size-large wp-image-4324\" src=\"http:\/\/bionauts.jp\/wp-content\/uploads\/2019\/05\/KJ-02501-1024x215.png\" alt=\"\" width=\"840\" height=\"176\" srcset=\"https:\/\/bionauts.jp\/wp-content\/uploads\/2019\/05\/KJ-02501-1024x215.png 1024w, https:\/\/bionauts.jp\/wp-content\/uploads\/2019\/05\/KJ-02501-300x63.png 300w, https:\/\/bionauts.jp\/wp-content\/uploads\/2019\/05\/KJ-02501-768x162.png 768w, https:\/\/bionauts.jp\/wp-content\/uploads\/2019\/05\/KJ-02501-1536x323.png 1536w, https:\/\/bionauts.jp\/wp-content\/uploads\/2019\/05\/KJ-02501-1200x252.png 1200w, https:\/\/bionauts.jp\/wp-content\/uploads\/2019\/05\/KJ-02501.png 1944w\" sizes=\"(max-width: 709px) 85vw, (max-width: 909px) 67vw, (max-width: 1362px) 62vw, 840px\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>Data<\/h3>\n<ul>\n<li>TA4C-payloads are easily synthesized with 1-3 steps using previously reported methods.<\/li>\n<li>Half-life can be controlled from several hours to one week.<\/li>\n<li>Fluorescent dye-conjugated trastuzumab shows the same affinity as the original one and fluoresced on the Her2 expressing cell surface (fig.R).<\/li>\n<\/ul>\n<h3>Patent\/Publication<\/h3>\n<ul>\n<li>PCT\/JP2020\/008357; US, EP, CN, JP<\/li>\n<li><a href=\"https:\/\/doi.org\/10.1002\/cbic.201900692\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.1002\/cbic.201900692<\/a><\/li>\n<\/ul>\n<h3>Researchers<\/h3>\n<p>Prof. Akira Onoda (Hokkaido University)<\/p>\n<h3>Expectations<\/h3>\n<ul>\n<li>We are seeking companies to license and commercialize this technology.<\/li>\n<li>Samples for your evaluation are available under material transfer agreement (MTA).<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>Project No: KJ-02501<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>One Step N-Terminal Conjugation of Functional Compounds<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0},"categories":[1],"tags":[],"categories_of_inventions":[50,47,48,46],"disease_categories___indication":[51,52,53,54,89,55,56,57,58,59,60,65,66,61,62,63,67,64,68,69],"asset_type":[71,94,95,72],"development_phase":[],"medicine":[147,121],"other_than_medicine":[132,130],"material":[],"engineering":[],"software_it":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/1286"}],"collection":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1286"}],"version-history":[{"count":8,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/1286\/revisions"}],"predecessor-version":[{"id":4325,"href":"https:\/\/bionauts.jp\/index.php?rest_route=\/wp\/v2\/posts\/1286\/revisions\/4325"}],"wp:attachment":[{"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1286"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1286"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1286"},{"taxonomy":"categories_of_inventions","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories_of_inventions&post=1286"},{"taxonomy":"disease_categories___indication","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fdisease_categories___indication&post=1286"},{"taxonomy":"asset_type","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fasset_type&post=1286"},{"taxonomy":"development_phase","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fdevelopment_phase&post=1286"},{"taxonomy":"medicine","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedicine&post=1286"},{"taxonomy":"other_than_medicine","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fother_than_medicine&post=1286"},{"taxonomy":"material","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmaterial&post=1286"},{"taxonomy":"engineering","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fengineering&post=1286"},{"taxonomy":"software_it","embeddable":true,"href":"https:\/\/bionauts.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fsoftware_it&post=1286"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}