Rapid detection of Carbapenemase-Producing Enterobacteriaceae (CPE)

Carbapenemase activity in bacterial cell suspension can be measured rapidly.


  • Rapidity: Detection of CPE within 30 to 60 mins.
  • Objectivity: Carbapenemase activity is readily quantifiable
  • High sensitivity and specificity: OXA-type carbapenemase is also detectable
  • Low cost: Easily and simply manufacturing for a disposable kit or device


Carbapenem-resistant Enterobacteriaceae (CRE) can be classified in two groups; one is CP-CRE (CPE) which has plasmids coding carbapenemase gene, and the other is non-CP-CRE which acquires resistance to carbapenem by genomic mutation.
Rapid detection of CPEs is important for the appropriate therapeutic approach and infection control measures in clinical settings, however previous methods are not suitable for the reason described below.



This is a novel method to detect Carbapenemase-Producing Enterobacteriaceae (CPE) in bacterial cell suspension. Researchers established a novel method to detect carbapenemase activity in bacterial cell suspension by using absorbance measurement. Compared to conventional visually method, this invention can detect and quantify CPEs. Additionally, the method can decrease false negatives and false positives, and makes you evaluate in objectively and high sensitivity.
At demonstration examination using imipenem, degradation level of this antibiotics by bacteria can be detected from the absorbances of 297 nm and 350 nm. This technology is independent from species and properties, so this method can detect CPEs whose weak carbapenemase activity. This advantage is useful detection OXA-type which are widely disseminating in Europe and the Middle East.

For detail information, please see the related paper described below.


Dan Takeuchi, et al. “Establishment of a dualwavelength spectrophotometric method for analysing and detecting carbapenemase-producing Enterobacteriaceae” Sci. Rep. (2018) 8:15689.
DOI: 10.1038/s41598-018-33883-0


Dan TAKEUCHI, Yukihiro AKEDA, Shigeyuki HAMADA and Kazunori TOMONO (Osaka University)


Applied in PCT and published as WO 2018/181997 A1 (in Japanese).
Before national phase.


We are looking for companies to collaborate with the researchers and develop this technology to a kit/device to detect CPE further under the patent licensing.

Product No:  CD-01886

Other than Medicine


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