➢ This discovery is an option for new semi-dwarf genetic resources that do not rely on the sd1 gene to affect yield
➢ It has established an ACL1 mutant and can be used as a parent strain.
➢ It can also be applied to other monocotyledonous plants and crops such as corn and barley.
➢ It is expected to be applied to strawberries, turf, etc., because the ACE1 hyperfunction extends its stolon.
Background and Technology
Cereals are prone to lodging when the ears become heavier, so the cross-fertilization with a semi dwarf property has been conducted. SD1 mutant (sd1) is the only known semi-dwarfing gene in rice, and while many cultivars have been improved, sd1 is known to affect the yield. The new semi-dwarfing gene is also useful from the viewpoint.
The inventors have identified a factor (ACE1 gene) that controls stem elongation in response to gibberellin in Deepwater rice by comparing Paddy rice and Deepwater rice (FIG. A). ACL1 (ACE1-like 1), one of the ACE1 homologues, is involved in GA-responsive internode elongation in short stem rice, and ACL1 mutants showed about 10% dwarfishness (Figure B). In addition, ACE1 related genes have been confirmed to be present in monocotyledonous plants such as corn, barley, Brachypodium distachyon (cereal model plants), and also in Arabidopsis thaliana. It is expected to be applied not only to rice but also to other crops.
ACL1 deletion mutants created by gene editing using Crispr/Cas9 showed about 10% dwarfishness compared to WT (short-culmed variety)
Dr. Keisuke Nagai and Dr. Motoyuki Ashikari (Nagoya University)
We look for companies interested in developing varieties using ACE1-related genes. In terms of Rice evaluation, it is possible to provide mutant strains as presupposing the licensing. It is also possible to conduct collaborative research for varieties improvement using ACE1-related genes in grains such as barley, corn and also in crops such as strawberries.